By Rodolfo Paoletti, Dr. David Kritchevsky
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Lipid-depleted serum was prepared by extraction of whole serum with diethyl ether and was depleted in cholesterol and triglyceride but not phospholipid. Presumably this preparation included phospholipid vesicles which ab sorbed apolipoproteins, resembling that produced by diethyl ether treat ment of VLDL or chylomicrons. Serum albumin in combination with egg yolk lecithin, but neither alone, caused efflux of sterol from the leukocytes. In a recent study Stein et al. (1978) studied the effect of LCAT on removal of cholesterol from human skin fibroblasts and rat aortic smooth muscle cells by LDL and VLDL-deficient serum.
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Bersot, T. , and Mahley, R. W. (1976). J. Biol. Chem. 251, 2395. Blanchette-Mackie, E. , and Scow, R. O. (1971). J. Cell Biol. 51, 1. Blanchette-Mackie, E. , and Scow, R. O. (1973). J. Cell Biol. 58, 689. Blanchette-Mackie, E. , and Scow, R. O. (1976). J. Lipid Res. 17, 57. Blok, M. , van Deenen, L. L. , and de Gier, J. (1977). Biochim. Biophys. Ada 464, 509. Blomhoff, J. P. (1974). Scand. J. Gastroenterol. 9, 591. Blum, C. , Levy, R. , Goebel, R. , and Berman, M. (1977). J. Clin. Invest. 60, 795.
Advances in lipid research. Vol. 17 by Rodolfo Paoletti, Dr. David Kritchevsky