By J.H.U. Brown, J.F. Dickson
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Extra resources for Advances in Biomedical Engineering. Volume 4
If t h e compound is one whose structure is known and whose fragmentation under electron impact is t o be studied, or if t h e compound is an unknown whose structure is t o be determined, t h e next step is commonly to determine t h e elemental composition of each ion. T h i s is possible because each isotope has a unique mass defect, or difference of t h e mass from an integral value. Algorithms for determining elemental composition generally involve subtracting from t h e experimentally determined mass t h e masses of all combinations of atoms other t h a n carbon and hydrogen considered likely to be present, and determining if t h e r e mainder fits t h e mass of some C W H W within an experimental limit.
Recognized by characteristic ions. F o r example, T M S derivatives of d i h y drodiols formed as metabolites of p h e n y l - s u b s t i t u t e d drugs show an ion a t 191 amu. T h e mass spectrum of t h e T M S derivative of t h e dihydrodiol of p h é n o b a r b i t a l is shown in Fig. 2 3 ; t h e base p e a k is a t 191 amu. T h e s a m e ion is p r o m i n e n t in mass spectra of T M S derivatives of certain steroids containing three hydroxyl groups in t h e D ring, and for a few other t y p e s of compounds as well, b u t in this instance it serves as a significant identifying p e a k for dihydrodiols.
STILLWELL A porous stainless-steel disk separator was described by Copet and E v a n s (1970). , 1969). T h i s separator is reported to have some a d v a n t a g e s over other types in work with organic compounds of low molecular weight; however, it is not widely used. Another description is t h a t of H a w e s et al. (1969). T h e Teflon separator of Lipsky et al (1966) h a s a narrow range of t e m p e r a t u r e for satisfactory operation and is rarely used. Several novel separators, based on t h e selective diffusibility of h y d r o gen through palladium or palladium-silver alloys, were developed by Lucero (1971) and Lucero and H a l e y (1968).
Advances in Biomedical Engineering. Volume 4 by J.H.U. Brown, J.F. Dickson